primary antibodies against crt (Abcam)
Structured Review

Primary Antibodies Against Crt, supplied by Abcam, used in various techniques. Bioz Stars score: 96/100, based on 729 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary antibodies against crt/product/Abcam
Average 96 stars, based on 729 article reviews
Images
1) Product Images from "Anti-tumor immunity enhancement by photodynamic therapy with talaporfin sodium and anti-programmed death 1 antibody"
Article Title: Anti-tumor immunity enhancement by photodynamic therapy with talaporfin sodium and anti-programmed death 1 antibody
Journal: Molecular Therapy Oncolytics
doi: 10.1016/j.omto.2022.12.009
Figure Legend Snippet: Induction of damage-associated molecular patterns (DAMPs) by TS-PDT in vitro (A) Flow cytometric analysis of cell-surface calreticulin (CRT). Negative controls, without TS addition or irradiation, are shown in red. The treated groups are shown in blue. (B) CRT translocation from the endoplasmic reticulum (ER) to the cell surface assessed by immunofluorescence staining. Images were obtained using a confocal microscope (original magnification ×350; scale bar, 5 μm). (C) Extracellular release of high-mobility group protein B1 (HMGB1) induced by TS-PDT assessed by the enzyme-linked immunosorbent assay (ELISA) (n = 3). (D) HMGB1 translocation from the ER to the cell surface was assessed by immunofluorescence staining. Images were obtained using a confocal microscope (original magnification ×350; scale bar, 5 μm). (E) Heat-shock protein (HSP)-90 expression on the cell surface induced by TS-PDT assessed by flow cytometry. Negative controls, without TS addition nor irradiation, are shown in red. The treated groups are shown in blue. (F) Extracellular release of ATP induced by TS-PDT assessed by ELISA. ATP concentration in the culture medium after TS-PDT was detected by ELISA (n = 3).
Techniques Used: In Vitro, Irradiation, Translocation Assay, Immunofluorescence, Staining, Microscopy, Enzyme-linked Immunosorbent Assay, Expressing, Flow Cytometry, Concentration Assay
